Automated Enzymatic Assay

The ACE kinetic kit from BÜHLMANN is a CE-marked kit providing ready to use Substrate and highly stable Calibrator and Controls, offering a maximum convenience and accuracy. Reconstituted Calibrator and Controls provided with the kits are stable for 6 months at 2-8°C. The substrate has an onboard-stability up to 3 months. Different package sizes are available (2×50, 100 and 1200 tests). CE-marked application notes are available for most common clinical chemistry analyzers.

 

Angiotensin Converting Enzyme


In vivo, ACE catalyses the conversion of Angiotensin I to Angiotensin II and inactivates bradykinin during regulating blood pressure via Renin-Angiotensin-System.

Elevated levels of serum ACE have been measured in patients suffering from various disorders. They often indicate a poor prognosis or rapid progression of the disease. Elevated serum ACE levels are reported in granulomatous-inflammatory diseases such as Sarcoidosis and Mixed-Connective-Tissue Disease (MTCD), Nephropathies associated with Diabetes and Glomerulonephritis, Cardiovascular diseases such as left ventricular hypertrophy, brain and myocardial infarction.
The main application is Sarcoidosis. Other areas of application are Chronic Berylliosis and monitoring of compliance under ACE inhibitor therapy.

 

High Sensitive Methods


In cerebrospinal fluid (CSF) normal ACE activity is far below the detection limit of any commercially available ACE kinetic assay. BÜHLMANN offers a solution to reliably detect even this low ACE activity. The  ACE high sensitive assay (kinetic assay; order code: KK-ACF) with a detection limit of 1 U/L.

 

ACE KINETIK Product Information

Method Enzymatic assay
Time to Result 10 min (approx.)
Sample Type 25 µl serum
Sensitivity 12 ACE units
Order Code KK-ACK  100 tests
KK-ACK2  2×50 tests
KK-ACKX  1200 tests
CE, FDA

 

Sarcoidosis


Sarcoidosis is an inflammatory disease of unknown origin characterised by the formation of granulomas. The prevalence of this multisystem granulomatous disorder, which activates the immune system, varies between ethnic groups and geographic regions. In about 20% of the cases especially multi-organ involvement may become life-threatening. The chronic forms can last several years or even a life time.

Consequently, diagnostic tools are essential to closely monitor disease activity and to predict disease progression. ACE is valuable to assess the disease activity, especially in systemic Sarcoidosis. It is released by epitheloid cells and its serum level correlates significantly with the granuloma burden of the patient.

The positive predictive value of elevated ACE activity is estimated between 75-90%, the negative predicted value between 70-80%. An initial low ACE activity indicates a good prognosis.

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