GanglioCombi-Light includes frequent gangliosides such as GD1b, GQ1b and GM1. Patients may be analysed for antibodies against each of these gangliosides so to obtain an IgG and IgM “mini-profile” per patient.

 

 

 

A Microtiter plate of GanglioCombi-Light is coated as follows:

Fig 1a

As an example, a GanglioCombi-Light could be used as shown in Fig 1b. Doing an IgG and IgM profile per patient there is capacity for 12 patients per plate.

Fig 1b

anti-GM1 antibodies: anti-IgG GM1 antibodies are implicated in the pathogenesis of Guillain-Barré Syndrome (GBS) and anti-IgM GM1 antibodies in multifocal neuropathy (MMN) and motor neuron diseases (MND). anti-IgM GM1 antibodies may be present as monoclonal IgM paraprotein or as a polyclonal IgM. Polyclonal IgG may be detected in patients with motor neuropathies. High GM1 antibody titers may be detected in association with in Guillain-Barré Syndrome (GBS) following Campylobacter jejuni infection. The fact that GM1 is the only common Glycolipid antigen reactive with all of the GM1 antibodies found in motor nerve disorders suggests that it is the relevant target antigen in neural tissue.

Anti-GD1b antibodies: Reactivity against Gangliosides containing disialosyl groups, particularly GD1b, has been reported in isolated cases of acute and chronic idiopathic ataxic neuropathies (SAN).
The clinical picture comprises a chronic neuropathy with marked sensory ataxia and areflexia and with relatively preserved motor function in the limbs. In addition many patients have preserved motor function. When present in their entirety, these clinical features are described under the acronym CANOMAD (chronic, ataxic, neuropathy, opthalmoplegia, IgM paraprotein, cold agglutinins, disialosy antibodies).

Anti-GQ1b antibodies: Anti- IgG GQ1b antibodies are closely associated with Miller-Fisher syndrome (MFS), which is discussed to be a variant of Guillain-Barré syndrome. Up to 100% of MFS patients have these antibodies. Anti-GQ1b antibodies decay rapidly with clinical recovery and usually are not found in normal and disease control samples. The close serologic-clinical association make anti-GQ1b antibodies in suspected cases of MFS a useful diagnostic marker for physicians with therapeutic significance.
 

Product Information

	GanglioCOMBI-LIGHT
Method	ELISA
Time to results	4.5 h
Sample Type	20 µl serum (1:50)
Standard range	quantitative
Isotypes	IgG and gM
Order code	EK-GCL-GM 2x12 profiles
	CE

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