Easy, Routine Drug of Abuse screening
The only automated enzymatic assay for quantitative measurement of urine and serum gamma-hydroxybutyric acid. The assay can be applied on any clinical chemistry analyzer. Results are available within 10 minutes without additional sample pretreatment.
NEW - improved stability
The improved enzyme formulation results in a significantly better reagent stability after reconstitution.
- On board reagent storage at up to 15°C is possible now
- Reagents are stable on board up to 2 months
- Less re-calibrations are required now
This allows a more efficient and economic use of this easy to perform enzymatic assay, and opens the door to offer a real 24/7 service to your customer.
The quantitative detection of the abuse/intoxication of gamma-hydroxybutyric acid (GHB) also known as liquid Ecstasy is still a challenge in Toxicology.
To date, GHB is not detected by routine blood or urine drug screens. Its detection is virtually only possible with chromatographic methods. These methods are time-consuming and laborious since they often require derivatization of the drug and/or sample extraction. Therefore many cases involving GHB may be missed in hospitals and forensic institutes.
This situation causes a permanent under-estimation of the amount of this kind of severe intoxications or the inappropriate care of victims. Clearly, there is a requirement for simple and rapid analytical procedures to meet current increased demand for analysis.
The BÜHLMANN enzymatic GHB assay will allow a direct, quick and easy quantification of exogenous intake of GHB.
Principle of the Assay
GHB is converted to its metabolite by the action of GHB-specific recombinant enzyme and oxidized nicotinamide adenine dinucleotide (NAD+). The increase in absorbance at 340 nm resulting from the reduction of NAD+ into NADH is proportional to the amount of GHB in the sample. The enzymatic GHB assay is a three reagent test which can be applied on every clinical chemistry analyzer allowing these settings.
Several evaluations showed comparable sensitivity and a very high correlation to the well established but laborious GC-MS methods.
Enzymatic Assay - Patent pending
|Time to Result||~10 min|
|Sample Type||Urine / Serum <10 µl|
||Instruction for use*||
General product information
|Technical product information|
||PDF [200 KB]||PDF [285 KB]||PDF [360 KB]|
|deutsch||PDF [285 KB]||PDF [360 KB]|
* Multilingual document [EN, DE, FR, IT, ES]
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