The Flow CAST^® highsens is intended for the determination of basophil activation in patient whole blood samples by flow cytometry. Our unique marker combination - CCR3, CD63 and CD203c - combines the simple gating properties for basophil detection by CCR3 with the complete new mix of the best activation markers CD63 and CD203c in one using a single fluorescence detection for both. Due to its very simple protocol the first results can be obtained within one hour - perfect conditions for routine laboratories.

CCR3 as Basophil Marker
Basophils constitutively express the eotaxin receptor CCR3 allowing for a robust and accurate selection of cells. CCR3 is detected with a PE-fluorescence labelled monoclonal antibody. This gating of basophil cells is significantly easier than with any other commercially available BAT.

CD63 and CD203c as Activation Markers
Upon allergen contact, sensitised basophils degranulate and express the cell surface marker CD63. In addition, the surface marker CD203c is up-regulated. Both are detected by the tandem dye PE-DY647 labeled to a monoclonal antibody. CD63 and CD203c are currently the best-validated basophil activation marker directly linked to basophil degranulation. We offer the unique possibility to measure two markers in one test to obtain maximal test-readout for the most sensitive diagnosis.

Controls
The first control, anti-FcEpsilonRI, is a highly specific monoclonal antibody binding to IgE receptor. The second control, a tripetide (fMLP) activates the basphils by a non-immunological way.

Product Information

* Multilingual document [EN, DE, FR, IT, ES]

Main applications for the Flow CAST^®

The CAST^® assays can be used for the confirmation of any IgE mediated allergy with controversial results from classical testing (anamnese, skin testing, spec. IgE). Further information about specific fields of allergy ask for independent testing on Cellular Allergy.